Spatial Resolution Improvement of Scanning Microscopy Based on Thermal Lens Spectroscopy with a Total-Internal-Reflection Arrangement
نویسندگان
چکیده
منابع مشابه
Spatial resolution improvement of scanning microscopy based on thermal lens spectroscopy with a total-internal-reflection arrangement.
In scanning microscopy by total internal reflection with thermal lens spectroscopy, its spatial resolution depends on the distance between the sample and a converging lens, which corresponds to the objective lens in an ordinary optical microscope. It was found that the resolution was best when the signal induced by the thermal lens effect was maximum. The distance was precisely adjusted by moni...
متن کاملTwo-photon Scanning Fluorescence Microscopy under Total Internal Reflection
Two-photon fluorescence microscopy is a powerful method for many applications. Because of the ability of strong attenuation of the background fluorescence, total internal reflection fluorescence microscopy (TIRFM) has been the major technique in the fluorescence imaging and single molecule detections. Recently, we have developed a new type of TIRFM called scanning TIRFM (henceforth abbreviated ...
متن کاملA Thin Layer Imaging with the Total Internal Reflection Fluorescence Microscopy
Total internal reflection fluorescence microscopy (TIRFM) is an optical technique that allows imaging of a thin layer of the sample with a thickness of about 100-200 nm. It is used in science of cell biology to study cellular processes, especially near the membranes of living cells. This method is based on the total internal reflection phenomenon, where the evanescent wave is generated in the l...
متن کاملFast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging.
Total internal reflection fluorescence microscopy (TIRFM) is the method of choice to visualize a variety of cellular processes in particular events localized near the plasma membrane of live adherent cells. This imaging technique not relying on particular fluorescent probes provides a high sectioning capability. It is, however, restricted to a single plane. We present here a method based on a v...
متن کاملSingle-shot super-resolution total internal reflection fluorescence microscopy
20 We demonstrate a simple method for combining instant structured illumination microscopy (SIM) 21 with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF 22 (down to 115 +/13 nm) and enabling imaging frame rates up to 100 Hz over hundreds of time points. 23 We apply instant TIRF-SIM to multiple live samples, achieving rapid, high contrast super-r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2005
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.21.469